BMP expression in duck interdigital webbing: a reanalysis.

base pairs (bp) from the C1H4 gene segment into unrelated rearrangements of V186.2. In the sample from B220lo GC cells, only a single VH V23 rearrangement encoded the YYYGS motif in CDR3, and three rearrangements, all V186.2, were not in-frame. Two of these nonproductive (nP) rearrangements resulted from incomplete codons at the V-D-J junction, whereas the third contained a complex 215-bp insertion of DNA from the V186.2 and VH 165.1 gene segments. Two hybrid sequences were present: the 215-bp insert described earlier and an in-frame exchange of 30 bp from the C1H4 exon into a productive V186.2 rearrangement. 27. C. Miller and G. Kelsoe, J. Immunol. 155, 3377 (1995). 28. S. Han and G. Kelsoe, unpublished data. 29. A. Bakhshi et al., Proc. Natl. Acad. Sci. U.S.A. 84, 2396 (1987); Y. Tsujimoto, E. Louie, M. M. Bashir, C. M. Croce, Oncogene 2, 347 (1988); F. Cotter, C. Price, E. Zucca, B. D. Young, Blood 76, 131 (1990). 30. G. M. Griffiths, C. Berek, M. Kaartinen, C. Milstein, Nature 312, 271 (1984). 31. We thank J. Cerny and A. Perelson for their review of this manuscript. Supported in part by USPHS grants HL48722 and AI40227 (M.S.S.) and AI24335, AG10207, and AG13789 (G. K ). S.R.D. is a recipient of an Arthritis Foundation Postdoctoral Fellowship, M.S.S. acknowledges the support of a Leukemia Society Scholarship and G.K. received funding from the Jeanne M. and Joseph P. Sullivan Foundation and the Santa Fe Institute.